详细说明
概述
产品名称Anti-FUBP3抗体[EPR13174-61]
描述
兔单克隆抗体[EPR13174-61] to FUBP3
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human FUBP3 aa 400-550. The exact sequence is proprietary.
Database link: Q96I24Run BLAST with
Run BLAST with 
阳性对照
MCF7, U87-MG or HepG2 lysate, Human kidney clear cell carcinoma or colon tissue, HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13174-61
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Chromatin Binding Proteins
DNA / RNA binding
Epigenetics and Nuclear Signaling
Transcription
Transcription Factors
Epigenetics and Nuclear Signaling
Transcription
Cancer susceptibility
Proto-oncogenes
Epigenetics and Nuclear Signaling
Transcription
Polymerase associated factors
Pol II Transcription
TFIIH
Anti-FUBP3 antibody [EPR13174-61] 图像
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 [EPR13174-61] antibody (ab181122)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181122hc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 [EPR13174-61] antibody (ab181122)
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 [EPR13174-61] antibody (ab181122)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181122hc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 [EPR13174-61] antibody (ab181122)
Immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 [EPR13174-61] antibody (ab181122)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181122if1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 [EPR13174-61] antibody (ab181122)
Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling FUBP3 with ab181122 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
![Western blot - Anti-FUBP3 [EPR13174-61] antibody (ab181122)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181122wb2.jpg)
Western blot - Anti-FUBP3 [EPR13174-61] antibody (ab181122)
Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/5000 dilution + HepG2 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution![Western blot - Anti-FUBP3 [EPR13174-61] antibody (ab181122)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181122wb1.jpg)
Western blot - Anti-FUBP3 [EPR13174-61] antibody (ab181122)
All lanes : Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/20000 dilution
Lane 1 : MCF7 lysate
Lane 2 : U87-MG lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
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