详细说明
概述
产品名称Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1)抗体[EPR18184]
描述
兔单克隆抗体[EPR18184] to Histone H2A (phospho S1) + Histone H4 (phospho S1)
经测试应用Dot Blot,WB,IHC-P,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2A aa 1-100 (phospho S1). The exact sequence is proprietary.
Database link: P04908阳性对照
WB: HeLa treated with 1.5µg/ml Colcemid for 12 hours whole cell lysates. IHC-P: human colon, mouse stomach, rat colon, human cerebral cortex, mouse heart and rat brain tissues. ICC/IF: HeLa cells.
常规说明
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol纯度Protein A purified
克隆单克隆
克隆编号EPR18184
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H2A
Phosphorylated
Epigenetics and Nuclear Signaling
Histones
H4
Phosphorylated
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] 图像
![Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177309184.jpg)
Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Dot blot analysis of Histone H2A (phospho S1) peptide (Lane 1), unmodified Histone H2A peptide (Lane 2), Histone H4 (phospho S1) peptide (Lane 3), and unmodified Histone H4 peptide (Lane 4), labeled using ab177309 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 11, 14 kDa
Observed band size : 11,14 kDa
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177309ged.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177309 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.![Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab1773099PA.jpg)
Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
All batches of ab177309 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on human colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on neuron cells of human cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse heart tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (ab177309)
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on neuron cells of rat brain tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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