详细说明
概述
产品名称Anti-Kinesin 5C抗体[EPR16224-32]
描述
兔单克隆抗体[EPR16224-32] to Kinesin 5C
经测试应用ICC/IF,WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human Kinesin 5C aa 600-850. The exact sequence is proprietary.
Database link: O60282Run BLAST with
Run BLAST with 
阳性对照
Neuro-2a, Human fetal brain, SH-SY5Y, mouse brain, rat brain and C6 lysate. Human testis tissue. Mouse cerebral cortex tissue. SH-SY5Y cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR16224-32
同种型IgG
研究领域
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Motor Proteins
Kinesin
Anti-Kinesin 5C antibody [EPR16224-32] 图像
![Western blot - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab193352521.jpg)
Western blot - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)
All lanes : Anti-Kinesin 5C antibody [EPR16224-32] (ab193352) at 1/10000 dilution
Lane 1 : Neuro-2a lysate
Lane 2 : Human fetal brain lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 109 kDa
![Western blot - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab193352522.jpg)
Western blot - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)
All lanes : Anti-Kinesin 5C antibody [EPR16224-32] (ab193352) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 109 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab193352523.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)
Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling Kinesin 5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab193352524.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)
Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling Kinesin 5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab193352525.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Kinesin 5C antibody [EPR16224-32] (ab193352)
Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Kinesin 5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
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