详细说明
概述
产品名称Anti-MTH1抗体[EPR15934-50]
描述
兔单克隆抗体[EPR15934-50] to MTH1
经测试应用WB,IHC-P,IP,Flow Cyt,ICC/IF
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human MTH1 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P36639Run BLAST with
Run BLAST with 
阳性对照
WB: Jurkat whole cell lysate; Human fetal kidney and fetal thymus lysates. IHC-P: Human thymus and squamous cell carcinoma of lung tissues. ICC/IF: Jurkat and A549 cells. Flow Cyt: Jurkat cells. IP: Jurkat whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15934-50
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
DNA Damage & Repair
Base Excision Repair
Anti-MTH1 antibody [EPR15934-50] 图像
![Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832OWB.jpg)
Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)
Predicted band size : 23 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MTH1 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: A549 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab200832 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab200832 was shown to specifically react with MTH1 when MTH1 knockout samples were used. Wild-type and MTH1 knockout samples were subjected to SDS-PAGE. ab200832 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) diluted to 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.![Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832832.JPG)
Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)
Anti-MTH1 antibody [EPR15934-50] (ab200832) at 1/5000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 23 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 secondsBlocking/dilution Buffer: 5% NFDM/TBST.
The expression profile observed is consistent with the following literature PMID: 11296483.
![Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832322.JPG)
Western blot - Anti-MTH1 antibody [EPR15934-50] (ab200832)
All lanes : Anti-MTH1 antibody [EPR15934-50] (ab200832) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 23 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteBlocking/dilution Buffer: 5% NFDM/TBST.
The expression profile observed is consistent with the following literature PMID: 11296483.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTH1 antibody [EPR15934-50] (ab200832)
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling MTH1 with ab200832 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Human thymus tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832Hcb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTH1 antibody [EPR15934-50] (ab200832)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of lung tissue labeling MTH1 with ab200832 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Human squamous cell carcinoma of lung tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Flow Cytometry - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2008322FC.jpg)
Flow Cytometry - Anti-MTH1 antibody [EPR15934-50] (ab200832)
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MTH1 with ab200832 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-MTH1 antibody [EPR15934-50] (ab200832)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200832328.JPG)
Immunoprecipitation - Anti-MTH1 antibody [EPR15934-50] (ab200832)
MTH1 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200832 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10ug (Input). Lane 2: ab200832 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200832 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 3 seconds.
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