概述

• 产品名称Anti-HNF-4-alpha抗体[EPR3648]
     

• 描述

  兔单克隆抗体[EPR3648] to HNF-4-alpha

• 经测试应用WB,IHC-P,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HNF-4-alpha aa 1-100.

• 阳性对照    

• WB: HepG2, A549 and SW480 cell lysates. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR3648

• 同种型IgG

• 研究领域

• Metabolism

• Types of disease

• Heart disease

• Metabolism

• Types of disease

• Cancer

• Metabolism

• Types of disease

• Diabetes

• Metabolism

• Pathways and Processes

• Mitochondrial Metabolism

• Cytochromes

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Energy transfer pathways

• Energy Metabolism

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Lipid and lipoprotein metabolism

• Lipases

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Lipid and lipoprotein metabolism

• Cholesterol Metabolism

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Lipid and lipoprotein metabolism

• Lipid metabolism

• Cancer

• Cancer Metabolism

• Metabolic signaling pathway

• Metabolism of lipids and lipoproteins

• Developmental Biology

• Organogenesis

• Gut development

• Liver development

• Cardiovascular

• Atherosclerosis

• Diabetes associated

• Epigenetics and Nuclear Signaling

• Transcription

• Polymerase associated factors

• Pol II Transcription

• Other

• Signal Transduction

• Metabolism

• Energy Metabolism

• Cardiovascular

• Lipids / Lipoproteins

• Lipid Metabolism

• Cytochromes

• Cardiovascular

• Lipids / Lipoproteins

• Lipid Metabolism

• Cholesterol Metabolism

• <a javascript:void(0)>Cardiovascular

• Blood

• Coagulation

• Extrinsic

Anti-HNF-4-alpha antibody [EPR3648] 图像

• 

  Western blot - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     Anti-HNF-4-alpha antibody [EPR3648] (ab92378) at 1/2000 dilution (purified) + SW480 cell lysate at 20 µg
  
  Secondary
  Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 53 kDa
  Observed band size : 53 kDa
     

  Blocking and dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     Anti-HNF-4-alpha antibody [EPR3648] (ab92378) at 1/5000 dilution (purified) + HepG2 cell lysate at 10 µg
  
  Secondary
  Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 53 kDa
  Observed band size : 53 kDa
     

  Blocking and dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     All lanes : Anti-HNF-4-alpha antibody [EPR3648] (ab92378) at 1/1000 dilution (unpurified)
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : A549 cell lysate
  Lane 3 : SW480 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
  
  Predicted band size : 53 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HNF-4-alpha with purified ab92378 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue (A) and human kidney tissue (B) labelling HNF-4-aplha with unpurified ab92378 at a 1/100 dilution. Detection: DAB staining.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling HNF-4 with purified ab92378 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

  Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

  Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Immunocytochemistry/Immunfluorescence analysis of HepG2 cells labelling HNF-4-alpha with unpurified ab92378 at a 1/100 dilution.

   

• 

  Flow Cytometry - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Flow Cytometry analysis of HepG2 cells labelling HNF-4 with purified ab92378 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

   

• 

  Flow Cytometry - Anti-HNF-4-alpha antibody [EPR3648] (ab92378)          

     

  Overlay histogram showing HepG2 cells stained with unpurified ab92378 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab92378, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.