详细说明
概述
产品名称Anti-HNF-4-alpha抗体[EPR16885]
描述
兔单克隆抗体[EPR16885] to HNF-4-alpha
经测试应用ChIP,IHC-P,WB,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Mouse HNF-4-alpha aa 1-200. The exact sequence is proprietary.
Database link: P49698Run BLAST with
Run BLAST with 
阳性对照
WB: HepG2 and SW480 whole cell lysates; Human fetal liver, colon and fetal kidney lysates; mouse and rat liver lysates. IHC-P: Human liver, Human colon, mouse liver and rat colon tissues. IP: HepG2 whole cell extract. ChIP: HepG2 whole cell extract.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol纯度Protein A purified
克隆单克隆
克隆编号EPR16885
同种型IgG
研究领域
Metabolism
Types of disease
Heart disease
Metabolism
Types of disease
Diabetes
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Cytochromes
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipases
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins
Developmental Biology
Organogenesis
Gut development
Liver development
Cardiovascular
Atherosclerosis
Diabetes associated
Epigenetics and Nuclear Signaling
Transcription
Polymerase associated factors
Pol II Transcription
Other
Signal Transduction
Metabolism
Energy Metabolism
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cytochromes
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism
Cardiovascular
Blood
Coagulation
Extrinsic
Anti-HNF-4-alpha antibody [EPR16885] 图像
![Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604b-1.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Anti-HNF-4-alpha antibody [EPR16885] (ab181604) at 1/1000 dilution + Full length mouse HNF-4-gamma recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604b-2.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Anti-HNF-4-alpha antibody [EPR16885] (ab181604) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604b-3.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Anti-HNF-4-alpha antibody [EPR16885] (ab181604) at 1/1000 dilution + SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604b-4.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
All lanes : Anti-HNF-4-alpha antibody [EPR16885] (ab181604) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human colon lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 1 minuteThis antibody can recognize 6 isoforms in human. The predicted MW are 53KDa, 52 KDa, 47KDa, 56 KDa, 49 KDa and 44 KDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604b-5.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
All lanes : Anti-HNF-4-alpha antibody [EPR16885] (ab181604) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Exposure time : 15 secondsThis antibody can recognize 2 isoforms in mouse and rat. The predicted MW are 53KDa and 52 KDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
![ChIP - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604led.jpg)
ChIP - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Chromatin was prepared from HepG2 (Human liver hepatocellular carcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab181604 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
ABCC6_NC1 is negative control
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF-4-alpha with ab181604 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF-4-alpha with ab181604 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604HCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HNF-4-alpha with ab181604 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on mouse liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604HCd.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HNF-4-alpha with ab181604 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on epithelial cells of rat colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunoprecipitation - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181604-IP.jpg)
Immunoprecipitation - Anti-HNF-4-alpha antibody [EPR16885] (ab181604)
HNF-4 alpha was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell extract with ab181604 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab181604 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HepG2 whole cell extract 10 µg (Input).
Lane 2: ab181604 IP in HepG2 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181604 in HepG2 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
粤公网安备44196802000105号
400-6226-992