详细说明
概述
产品名称Anti-HNF-4-alpha抗体[EPR16885-99]
描述
兔单克隆抗体[EPR16885-99] to HNF-4-alpha
经测试应用WB,IHC-P,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Mouse HNF-4-alpha aa 1-200. The exact sequence is proprietary.
Database link: P49698Run BLAST with
Run BLAST with 
阳性对照
WB: HepG2 and Caco-2 whole cell lysates; Human colon and fetal kidney lysates. IHC-P: Human colon, Human liver, mouse liver and rat colon tissues. ICC/IF: HepG2 and HT-29 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16885-99
同种型IgG
研究领域
Metabolism
Types of disease
Heart disease
Metabolism
Types of disease
Diabetes
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Cytochromes
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipases
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins
Developmental Biology
Organogenesis
Gut development
Liver development
Cardiovascular
Atherosclerosis
Diabetes associated
Epigenetics and Nuclear Signaling
Transcription
Polymerase associated factors
Pol II Transcription
Other
Signal Transduction
Metabolism
Energy Metabolism
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cytochromes
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism
Cardiovascular
Blood
Coagulation
Extrinsic
Anti-HNF-4-alpha antibody [EPR16885-99] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460t29.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1: ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2: ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460hep.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1: ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2: ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.![Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460WBa.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution + Full length mouse HNF-4-gamma recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Based on sequence analysis of the immunogen, ab201460 shares 72% homology with HNF4 gamma. The levels of XR were tested in the accompanying recombinant protein, WB experiment.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460WBb.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460WBc.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution + Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460WBd.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
All lanes : Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution
Lane 1 : Human colon lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460HCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201460HCd.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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