详细说明
概述
产品名称Anti-NEDD4-2 (phospho S448)抗体[EPR8269(2)]
描述
兔单克隆抗体[EPR8269(2)] to NEDD4-2 (phospho S448)
经测试应用WB,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448.
Database link: Q96PU5阳性对照
293T cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.
If you have any questions regarding this update, please contact our Scientific Support team.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR8269(2)
同种型IgG
研究领域
Cell Biology
Proteolysis / Ubiquitin
Proteasome / Ubiquitin
Ubiquitin E3 Enzymes
Hect E3 Ligase
Epigenetics and Nuclear Signaling
Chromatin Modifying Enzymes
Ubiquitylation
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] 图像
![Immunoprecipitation - Anti-NEDD4-2 (phospho S448) [EPR8269(2)] antibody (ab168349)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16834949b.jpg)
Immunoprecipitation - Anti-NEDD4-2 (phospho S448) [EPR8269(2)] antibody (ab168349)
Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.![Western blot - Anti-NEDD4-2 (phospho S448) [EPR8269(2)] antibody (ab168349)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab16/ab16834949a.jpg)
Western blot - Anti-NEDD4-2 (phospho S448) [EPR8269(2)] antibody (ab168349)
All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size : 112 kDa
Additional bands at : 110-135 kDa. We are unsure as to the identity of these extra bands.
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