Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Benefits  

• Rolls out of bottle for easy cell culture medium preparation

• Eliminates time spent aliquoting

• One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?  

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

What are ProDots® Proteins?  

ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.

• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S.     et al. (1989) Proc. Natl. Acad. Sci. USA     86:802. The ED    ₅₀ for this effect is 20-100 ng/mL.

• Source

  E. coli-derived Cys37 - Ser208 & Gly41 - Ser208

• Accession #

• N-terminal Sequence    
  Analysis

  Cys37 & Gly41

• Predicted Molecular Mass

  19.5 kDa

Data Images

Background: FGF-10

FGF-10 (Fibroblast Growth Factor 10) is a heparin binding glycoprotein in the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer.

• Long Name:

  Fibroblast Growth Factor 10

• Entrez Gene IDs:

  2255 (Human); 14165 (Mouse); 25443 (Rat)

• Alternate Names:

  FGF10; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; KGF-2; produced by fibroblasts of urinary bladder lamina propria