概述

• 产品名称Anti-P Glycoprotein抗体[EPR10364-57]
     

• 描述

  兔单克隆抗体[EPR10364-57] to P Glycoprotein

• 特异性P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab170904 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein.

• 经测试应用IHC-FoFr,IHC-P,WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Recombinant fragment within Human P Glycoprotein aa 350-750. The exact sequence is proprietary.
  Database link: P08183

• 阳性对照    

• WB: 293T, HeLa, HepG2 and 293T cell lysates; human fetal brain tissue lysate; mouse brain, heart, kidney and spleen tissue lysates; rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human hepatocellular carcinoma, brain and kidney tissues.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

  If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

   

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR10364-57

• 同种型IgG

• 研究领域

• Cancer

• Drug resistance

• P glycoproteins

• Stem Cells

• Hematopoietic Progenitors

• Surface Molecules

• Stem Cells

• Hematopoietic Progenitors

• Side Population

• Signal Transduction

• Metabolism

• Plasma Membrane

• Channels

• Signal Transduction

• Metabolism

• Plasma Membrane

• ATPases

Anti-P Glycoprotein antibody [EPR10364-57] 图像

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)
  
  Lane 1 : mouse brain lysate
  Lane 2 : C6 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST
  Dilution buffer: 5% NFDM/TBST

   

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (purified)
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HepG2 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST
  Dilution buffer: 5% NFDM/TBST

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.  

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution (unpurified)
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : Mouse brain tissue lysate
  Lane 3 : Rat brain tissue lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM/TBST.

   

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : Mouse brain tissue lysate
  Lane 3 : Rat brain tissue lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM/TBST.

   

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : 293T cell lysate
  Lane 4 : Human fetal brain tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Lanes 1 - 3 : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
  Lane 4 : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
  
  developed using the ECL technique
  
  Predicted band size : 141 kDa
   

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
  
  Lane 1 : Rat brain tissue lysate
  Lane 2 : Rat heart tissue lysate
  Lane 3 : Rat kidney tissue lysate
  Lane 4 : Rat spleen tissue lysate
  
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 2 minutes  

• 

  Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
  
  Lane 1 : Mouse brain tissue lysate
  Lane 2 : Mouse heart tissue lysate
  Lane 3 : Mouse kidney tissue lysate
  Lane 4 : Mouse spleen tissue lysate
  
  Secondary
  HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution
  developed using the ECL technique
  
  Predicted band size : 141 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 2 minutes  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling P Glycoprotein with unpurified ab170904 at 1/250 dilution.