概述

• 产品名称Anti-Nucleophosmin (phospho S125)抗体[EPR1856]
     

• 描述

  兔单克隆抗体[EPR1856] to Nucleophosmin (phospho S125)

• 特异性Detects Nucleophosmin only if phosphorylated at Serine 125.

• 经测试应用Flow Cyt,WB,IP,IHC-P,ICC

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Phospho-peptide corresponding to residues surrounding Serine 125 of Human Nucleophosmin

• 阳性对照    

• WB: HeLa cell lysate IHC-P: Human breast or kidney tissue

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR1856

• 同种型IgG

• 研究领域

• <a javascript:void(0)>Epigenetics and Nuclear Signaling

• DNA / RNA

• Translation

• Regulation

Anti-Nucleophosmin (phospho S125) antibody [EPR1856] 图像

• 

  Flow Cytometry - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)          

     Overlay histogram showing HeLa cells stained with ab109546 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109546, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.  

• 

  Western blot - Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)          

     All lanes : Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) at 1/10000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 33 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)          

     ab109546, at a 1/100 dilution, staining Nucleophosmin in formailn-fixed, paraffin-embedded Human breast tissue.  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)          

     ab109546, at a 1/100 dilution, staining Nucleophosmin in formalin-fixed, paraffin-embedded Human kidney tissue.