概述

• 产品名称Anti-Nucleophosmin (phospho T199)抗体[EP1857Y]
     

• 描述

  兔单克隆抗体[EP1857Y] to Nucleophosmin (phospho T199)

• 特异性Recognizes Nucleophosmin only when phosphorylated at T199.

• 经测试应用WB,IHC-P,ICC/IF,IP,Flow Cyt

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic phosphopeptide corresponding to residues surrounding Theonine 199 of Human Nucleophosmin

• 阳性对照    

• WB: lysate from HeLa cell treated with CA; IHC-P: Human lymphoma; ICC/IF: HeLa cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP1857Y

• 同种型IgG

• 研究领域

• <a javascript:void(0)>Epigenetics and Nuclear Signaling

• DNA / RNA

• Translation

• Regulation

Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] 图像

• 

  Western blot - Nucleophosmin (phospho Y199) antibody [EP1857Y] (ab81551)          

     All lanes : Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551) at 1/2500 dilution
  
  Lane 1 : lysate from untreated HeLa cells
  Lane 2 : lysate from HeLa cells treated with CA
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled Goat anti-Rabbit at 1/2000 dilution
  
  Predicted band size : 33 kDa
  Observed band size : 33 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleophosmin (phospho Y199) antibody [EP1857Y] (ab81551)          

     Immunohistochemistry analysis of paraffin-embedded Human lymphoma tissue, using 1/100 ab81551.  

• 

  Immunocytochemistry/ Immunofluorescence - Nucleophosmin (phospho Y199) antibody [EP1857Y] (ab81551)          

     Immunofluorescent staing of HeLa cells using 1/100 ab81551.  

• 

  Immunocytochemistry/ Immunofluorescence - Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551)      This image is a courtesy of Anonymous Abreview    

     

  ab81551 staining Nucleophosmin (phospho T199) in human HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton x100 in PBS and blocking with 5% serum was performed for 30 minutes at 23⁰C. Samples were incubated with primary antibody (1/150 in PBS) for 1 hour at 23°C. An Alexa Fluor^®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.

     

  See Abreview

• 

  Flow Cytometry-Anti-Nucleophosmin (phospho T199) antibody [EP1857Y](ab81551)          

     Overlay histogram showing HeLa cells stained with ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.