详细说明
概述
产品名称Anti-p38 (phospho T180 + Y182)抗体[EPR18120]
描述
兔单克隆抗体[EPR18120] to p38 (phospho T180 + Y182)
经测试应用WB,Dot Blot
种属反应性
与反应: Mouse, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p38 aa 150-250 (phospho T180 + Y182). The exact sequence is proprietary.
Database link: Q16539阳性对照
WB: HEK-293 whole cell lysate treated with UV (60 J/M2) for 60 minutes; NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR18120
同种型IgG
研究领域
Immunology
Innate Immunity
TLR Signaling
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Anti-p38 (phospho T180 + Y182) antibody [EPR18120] 图像
![Dot Blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195049b-1.jpg)
Dot Blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)
Dot blot analysis of p38 (phospho T180) peptide (Lane 1), p38 (phospho Y182) (Lane 2), p38 (phospho T180 + Y182) peptide (Lane 3), and non-phospho peptide (Lane 4), labeled using ab195049 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195049b-2.jpg)
Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)
All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195049b-3.jpg)
Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)
All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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