• Species Reactivity

  Human

• Specificity

  Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137    
  Leu24-Gln186    
  Accession # Q07011

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• Immunohistochemistry

  5-25 µg/mL

  See below

• 
  

• Agonist Activity

  Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3.    
  The ED    ₅₀ for this effect is typically 5 - 20 μg/mL.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  5-15 µg/mL

  Immersion fixed human peripheral blood mononuclear cells

• 
  

• Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Human 4‑1BB/TNFRSF9/CD137 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

• References:

1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.

2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.

• Entrez Gene IDs:

  3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

• Alternate Names:

  4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9