概述

• 产品名称Anti-Pirh2抗体[EPR18553]
     

• 描述

  兔单克隆抗体[EPR18553] to Pirh2

• 经测试应用ICC/IF,IP,WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

• 阳性对照    

• WB: HEK-293, HCT 116, LNCaP, RAW 264.7 and NIH/3T3 whole cell lysates; Human fetal heart, fetal kidney and fetal spleen lysates; Mouse kidney and spleen lysates. ICC/IF: HeLa and HEK-293 cells. IP: HeLa whole cell lysate.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR18553

• 同种型IgG

• 研究领域

• Cancer

• Cell cycle

• Cell cycle inhibitors

• p53 pathway

• <a javascript:void(0)>Cell Biology

• Proteolysis / Ubiquitin

• Proteasome / Ubiquitin

• Ubiquitin

Anti-Pirh2 antibody [EPR18553] 图像

• 

  Western blot - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
  
  Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
  Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
  Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 30 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
  
  Lane 1 : Human fetal heart lysate
  Lane 2 : Human fetal kidney lysate
  Lane 3 : Human fetal heart lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 30 kDa
  
  
  Exposure time : 30 seconds    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
  
  Lane 1 : Mouse kidney lysate
  Lane 2 : Mouse spleen lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 30 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
  
  Lane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
  Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 30 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Pirh2 with ab189907 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

  Confocal image showing nuclear and cytoplasmic staining on HeLa cell line.

  The nuclear counterstain is DAPI (blue).

  Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

  The negative controls are as follows:-

  -ve control 1: ab189907 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

  -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling Pirh2 with ab189907 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

  Confocal image showing nuclear and cytoplasmic staining on HEK-293 cell line.

  The nuclear counterstain is DAPI (blue).

  Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

  The negative controls are as follows:-

  -ve control 1: ab189907 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

  -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

   

• 

  Immunoprecipitation - Anti-Pirh2 antibody [EPR18553] (ab189907)          

     

  Pirh2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab189907 at 1/50 dilution.

  Western blot was performed from the immunoprecipitate using ab189907 at 1/1000 dilution.

  VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

  Lane 1: HeLa whole cell lysate 10ug (Input).

  Lane 2: ab189907 IP in HeLa whole cell lysate.

  Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189907 in HeLa whole cell lysate.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 30 seconds.