• Species Reactivity

  Human

• Specificity

  Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Methionine Aminopeptidase 2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 405419

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Methionine Aminopeptidase 1/METAP1    
  His52-Phe386    
  Accession # P53582

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Methionine Aminopeptidase 1 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 1/METAP1

The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The amino acid sequence of human METAP1 is 99%, 98%, 95% and 64% identical to that of chimpanzee, rat/mouse, chicken and fruit fly.

• References:

1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.

2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.

3. Liu, S. et al. (1998) Science 282:1324.

• Entrez Gene IDs:

  23173 (Human); 75624 (Mouse)

• Alternate Names:

  DKFZp781C0419; EC 3.4.11.18; KIAA0094MAP 1; MAP1; MAP1A; MetAP 1; MetAP1; MetAP1A; Methionine Aminopeptidase 1; methionyl aminopeptidase 1; Peptidase M 1