• Species Reactivity

  Human

• Specificity

  Detects human Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B3, C1, D1, or recombinant mouse Plexin A1, A2, or A3 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 537237

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2    
  Leu20-Arg1160    
  Accession # O15031

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Plexin B2 under non-reducing conditions only

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B2

Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha -chain (aa 20‑1164) and an 80 kDa TM beta ‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

• Entrez Gene IDs:

  23654 (Human); 140570 (Mouse); 315217 (Rat)

• Alternate Names:

  MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2