• Species Reactivity

  Human

• Specificity

  Detects human Protein Disulfide Isomerase/P4HB in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 537331

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Protein Disulfide Isomerase/P4HB    
  Asp18-Lys505    
  Accession # P07237

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1-2 µg/mL

  See below

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human P4HB (Catalog # ),

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein Disulfide Isomerase/P4HB

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family.  As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase.  This catalyses the hydroxylation of proline in collagen.  On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells.  Mature human P4HB is 491 amino acids (aa) in length.  It contains two TRX domains (aa 25-134 and 368-475) plus an ER retention sequence (aa 505-508).  There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18-505, human P4HB shares 94% aa identity with mouse P4HB. 

• Long Name:

  Proline 4-Hydroxylase, Beta Polypeptide

• Entrez Gene IDs:

  5034 (Human); 18453 (Mouse); 25506 (Rat)

• Alternate Names:

  Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1); PDIEC 5.3.4.1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide; PROHB; PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; Thbp; thyroid hormone-binding protein p55