• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat 14-3-3 gamma in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 505810

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human 14-3-3 gamma    
  Met1-Asn247    
  Accession # P61981

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 gamma

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ. 14-3-3 gamma becomes phosphorylated in response to PDGF and subsequently interacts with different PKC isoforms.

• Entrez Gene IDs:

  7532 (Human); 22628 (Mouse); 56010 (Rat)

• Alternate Names:

  1433 gamma; 14-3-3 gamma; 14-3-3 protein gamma; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide; YWHAG