• Species Reactivity

  Feline

• Specificity

  Detects feline IL‑10 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse, cotton rat, porcine, rat, equine, or human IL-10 is observed, and approximately 15% cross-reactivity with recombinant canine IL-10 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 137705

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant feline IL‑10    
  Ser19-Ile178    
  Accession # P55029

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Feline IL-10 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Feline IL‑10 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Feline IL‑10 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Feline IL-10 Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The feline homologue of murine IL-10 has been cloned. Feline IL-10 shares 78% amino acid sequence homology with human IL-10 and 73% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

• References:

1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

• Long Name:

  Interleukin 10

• Entrez Gene IDs:

  3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)

• Alternate Names:

  CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF