• Species Reactivity

  Human

• Specificity

  Detects human MMP‑25/MT6-MMP in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 141811

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human MMP‑25/MT6-MMP    
  Pro314-Cys508    
  Accession # Q9NPA2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-25/MT6-MMP

MMP-25, also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. MMP-25 cleaves collagen type 4, gelatin, fibronectin, and fibrin.

• Long Name:

  Matrix Metalloproteinase 25/Membrane Type 6 MMP

• Entrez Gene IDs:

  64386 (Human)

• Alternate Names:

  Leukolysin; matrix metallopeptidase 25; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-25; matrix metalloproteinase-like 1; membrane-type 6 matrix metalloproteinase; membrane-type matrix metalloproteinase 6; membrane-type-6 matrix metalloproteinase; MMP20; MMP20A; MMP25; MMP-25; MMPL1; MT6MMP; MT6-MMP; MT-MMP 6; MTMMP6; MT-MMP6