• Species Reactivity

  Mouse

• Specificity

  Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Lefty A, recombinant mouse (rm) ART, rmCripto, rhCripto-1, recombinant drosophila DPP, recombinant rat (rr) GDNF, rhLAP, rmLefty-1, rrMIS, rhPersephin, rmNodal, recombinant amphibian TGF-beta 5, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, rhTGF-alpha, or rhNeurturin is observed.

• Source

  Monoclonal Rat IgG    ₁ Clone # 185230

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse Artemin    
  Ala112-Gly224    
  Accession # Q9Z0L2.1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Artemin (Catalog # )    
  under non-reducing conditions only

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Artemin

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF‑ beta ) superfamily (1‑4). Similar to other TGF‑ beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide‑linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1‑GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase) (5‑8). Artemin prefers to bind to GFR alpha 3 and activites the GFR alpha 3‑RET. However, in the presence of RET, it can bind to GFR alpha 1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

• References:

1. Lin, L-F.H. et al. (1993) Science 260:1130.

2. Milbrandt, J. et al. (1998) Neuron 20:245.

3. Kotzbauer, P.T. et al. (1996) Nature 384:467.

4. Baloh, R.H. et al. (1998) Neuron 21:1291.

5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.

6. Baloh, R.H. et al. (1997) Neuron 18:793.

7. Jing, S. et al. (1996) Cell 85:1113.

8. Jing, S. et al. (1997) J Biol Chem 272:33111.

9. Nishino, J. et al. (1999) Neuron 23:725.

10. Enomoto, H. et al. (2001) Development 128:3963.

11. Andres, R. et al. (2001) Development 128:3685.

• Entrez Gene IDs:

  9048 (Human); 11876 (Mouse)

• Alternate Names:

  Artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin