• Species Reactivity

  Human

• Specificity

  Detects human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Granzyme D or rmGranzyme G is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 185813

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Granzyme H    
  Glu19-Leu246    
  Accession # P20718

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Granzyme H (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme H

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). After being activated by active cathepsin C, human Granzyme H cleaves a thioester substrate (8).

• References:

1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.

2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.

3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.

4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.

5. Meier, M. et al. (1990) Biochemistry 29:4042.

6. Haddad, P. et al. (1991) Int. Immunol. 3:57.

7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.

8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

• Entrez Gene IDs:

  2999 (Human)

• Alternate Names:

  Cathepsin G-like 2; CCP-X; CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1; CGL2; CGL-2; CSP-Ccytotoxin serine protease-C; CTLA1; CTSGL2; CTSGL2granzyme H; Cytotoxic serine protease C; Cytotoxic T-lymphocyte proteinase; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.79; granzyme H (cathepsin G-like 2, protein h-CCPX); Granzyme H; GrzH; GZMH