• Species Reactivity

  Human

• Specificity

  Detects human ADAMTS15 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 561819

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS15    
  Gly18-Cys682    
  Accession # Q8TE58

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS15

A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) is a secreted multi-domain protease that is primarily expressed in fetal liver and kidney. ADAMTS1, 4, 5, 8, and 15 form a subfamily of ADAMTS proteases that possess aggrecanase activity. These proteins are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. ADAMTS15 functions as a suppressor of tumor growth and invasion. It is downregulated in colon cancer, and its expression in breast cancer correlates with poor prognosis. Within amino acids (aa) 18-682 (which includes the propeptide, peptidase, disintegrin, first TSP-1 and most of the Cys-rich domain), human ADAMTS15 shares 95% aa sequence identity with mouse and rat ADAMTS15.

• Long Name:

  A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

• Entrez Gene IDs:

  170689 (Human); 235130 (Mouse); 300474 (Rat)

• Alternate Names:

  A disintegrin and metalloproteinase with thrombospondin motifs 15; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein; ADAM metallopeptidase with thrombospondin type 1 motif, 15; ADAM-TS 15; ADAMTS15; ADAM-TS15; ADAMTS-15; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82; metalloprotease disintegrin 15 with thrombospondin domains; MGC126403