• Species Reactivity

  Human

• Specificity

  Detects human IL-34 in sandwich immunoassays.  In this format, no cross-reactivity with recombinant mouse IL-34 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 578410

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL-34    
  Asn21-Pro242    
  Accession # Q6ZMJ4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human IL-34 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human IL‑34 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 µg/mL 

  Human IL‑34 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human IL-34 Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. The mature chain contains one potential site for N-linked glycosylation. IL-34 is widely expressed, but it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R). Functionally, IL-34 stimulates monocyte viability.

• Long Name:

  Interleukin 34

• Entrez Gene IDs:

  146433 (Human); 76527 (Mouse)

• Alternate Names:

  C16orf77; IL34; IL-34