• Species Reactivity

  Human

• Specificity

  Detects the pro region of the human BMP-2 precursor in direct ELISAs and Western blots. This antibody does not recognize mature recombinant human BMP‑2.

• Source

  Monoclonal Mouse IgG    _2A Clone # 253717

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human BMP‑2    
  Ala22-Lys281 (Arg245Ser, Arg279Ser)    
  Accession # P12643

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human BMP‑2 (Pro Region)

• 
  

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2

BMP-2, a member of the transforming growth factor beta (TGF-beta ) superfamily, is synthesized as an inactive precursor protein. The N-terminal pro region is removed via proteolytic cleavage to liberate a free 114 amino acid C-terminal fragment that forms a disulfide-linked active BMP-2 homodimer. BMP-2 modulates growth and differentiation of many cell types through its interaction with heterodimeric receptors composed of BMPR-II complexed with BMPR-IA or -IB.

• Long Name:

  Bone Morphogenetic Protein 2

• Entrez Gene IDs:

  650 (Human); 12156 (Mouse); 29373 (Rat)

• Alternate Names:

  BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2