• Species Reactivity

  Human

• Specificity

  Detects human Integrin  beta 6 in direct ELISAs and Western blots. In Western blots (under reducing conditions), 40% cross‑reactivity with recombinant human (rh) Integrin  beta 2 is observed and no cross-reactivity with rhIntegrin beta 1, 3, 5, 7, or 8 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 437216

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Integrin  beta 6    
  Gly22-Asn707    
  Accession # P18564

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Integrin  beta 6

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and porcine integrin beta 6 ECD, respectively.

• Entrez Gene IDs:

  3694 (Human); 16420 (Mouse); 311061 (Rat)

• Alternate Names:

  Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6