• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse TREM-2 in direct ELISAs and Western blots. Stains TREM-2 transfectants but not TREM-1 transfectants.

• Source

  Monoclonal Rat IgG    _2B Clone # 237920

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TREM-2    
  extracellular domain    
  Accession # Q99NH8

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-2

TREM-2 is a single V-type Ig-like domain-containing transmembrane (TM) receptor that is expressed on cells of the hematopoietic and nervous systems. Although single residue polymorphisms may exist, one soluble isoform variant is reported that shows an 88 amino acid (aa) substitution for the C-terminal 66 aa of the TM isoform. TREM-2 is best known for its association with the adapter protein DAP12 that delivers an activating signal that plays a role in both the innate and adaptive immune responses.

• Long Name:

  Triggering Receptor Expressed on Myeloid Cells 2

• Entrez Gene IDs:

  54209 (Human)

• Alternate Names:

  TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2