• Species Reactivity

  Human

• Specificity

  Detects human Airway Trypsin-like Protease/HAT in direct ELISAs and Western blots. Recognizes the catalytic-domain of recombinant human HAT.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 337029

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Airway Trypsin-like Protease/HAT    
  Ala42-Ile418    
  Accession # O60235

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Airway Trypsin-like Protease/HAT (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Airway Trypsin-like Protease/HAT

Human Airway Trypsin‑like protease (HAT), also known as Transmembrane Protease, Serine 11D (TMPRSSIID), is a type II transmembrane serine protease that is expressed in the cells of the submucosal serous glands of the bronchi and trachea (1‑3). The deduced sequence predicts a short cytoplasmic tail (aa 120), a transmembrane domain (aa 21‑41), and an ectodomain consisting of a SEA domain (aa 44‑164) and a catalytic domain (aa 187‑418). The single‑chain precursor can be converted into a disulfide‑linked two‑chains, one of which corresponds to the catalytic domain. HAT was initially purified from the sputum of patients with chronic airway diseases (1). HAT has been shown to induce PAR‑2 mediated IL‑8 release in psoriasis vulgaris and increase mucin expression in airway epithelial cells (4, 5). The amino acid sequence of human HAT is 99%, 79%, and 67% identical to that of chimpanzee, canine and rat/mouse.

• References:

1. Yasuoka, S. et al. (1997) Am. J. Respir. Cell Mol. Biol. 16:300.

2. Yamaoka, K. et al. (1998) J. Biol. Chem. 273:11895.

3. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.

4. Iwakiri, K. et al. (2004) J. Invest. Dermatol. 122:937.

5. Chokki, M. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:470.

• Long Name:

  Human Airway Trypsin-like Protease

• Entrez Gene IDs:

  9407 (Human)

• Alternate Names:

  airway trypsin like protease; Airway trypsin-like protease; EC 3.4.21; EC 3.4.21.-; HAT; MGC150587; MGC150588; TMPRSS11D; transmembrane protease serine 11D; transmembrane protease, serine 11D