• Species Reactivity

  Canine, Feline

• Specificity

  Detects canine and feline SCF/c‑kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50%‑100% cross‑reactivity with recombinant human (rh) SCF and recombinant mouse SCF is observed and no cross-reactivity with rhM-CSF is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 371907

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant canine SCF/c‑kit Ligand    
  Lys26-Ala190    
  Accession # Q06220

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Canine SCF/c-kit Ligand (Catalog # )    
  Recombinant Feline SCF/c-kit Ligand (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

SCF, also known as c-kit ligand, is a 28 kDa‑40 kDa type I transmembrane (TM) glycoprotein that plays important roles in fetal and adult developmental processes. Endothelial cells, fibroblasts, and keratinocytes can express SCF. The 190 aa extracellular region contains a proteolytic cleavage site, generating 165 aa circulating monomers and noncovalent homodimers. Soluble feline and canine SCF share 93% aa identity with each other and 88%‑92% aa identity with pig, cow, sheep, goat, horse, and mink SCF.

• Long Name:

  Stem Cell Factor

• Entrez Gene IDs:

  4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

• Alternate Names:

  c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor