• Species Reactivity

  Human, Rat

• Specificity

  Detects human and rat MafB at in Western blots. No cross-reactivity with recombinant human (rh) MafF, rhMafG, or rhMafK is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 372239

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human MafB    
  Met1-Leu323    
  Accession # Q9Y5Q3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Simple Western

  5 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafB

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. MafB has been implicated in the regulation of hindbrain development.

• Long Name:

  v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B

• Entrez Gene IDs:

  9935 (Human); 16658 (Mouse)

• Alternate Names:

  Kreisler (mouse) maf-related leucine zipper homolog; Kreisler maf-related leucine zipper homolog; KRML; KRMLMAFB/Kreisler basic region/leucine zipper transcription factor; MafB; Maf-B; MGC43127; transcription factor MafB; v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog B