• Species Reactivity

  Human

• Specificity

  Detects human Rex-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human YY1.

• Source

  Monoclonal Rat IgG    _2A Clone # 373722

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Rex-1    
  Ser2-Lys150    
  Accession # NP_777560

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Rex‑1

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rex-1/ZFP42

Human Rex-1 (also Zfp-42) is a 35 kDa zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa 214-307. Over this region, human and mouse show modest orthology at 47% aa identity.

• Long Name:

  Zinc Finger Protein 42

• Entrez Gene IDs:

  132625 (Human); 22702 (Mouse); 290765 (Rat)

• Alternate Names:

  FLJ20086; KIAA1584; MGC21637; MGC61687; Rex1; Rex-1; SUHW4; Suppressor of hairy wing homolog 4; ZFP42; zinc finger protein 280D; Zinc finger protein 634; ZNF634suppressor of hairy wing homolog 4 (Drosophila)