• Species Reactivity

  Human

• Specificity

  Detects human NFAM1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 374301

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human NFAM1    
  Gln43-Lys163    
  Accession # Q8NET5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human NFAM1

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFAM1

NFAM1 is a ~30 kDa transmembrane ITAM-containing glycoprotein of the Ig superfamily that activates the NFAT family of transcription factors. NFAM1 is expressed in leukocytes, predominantly splenic B and T cells, and is involved in B cell activation and signaling. The 121 aa human NFAM1 extracellular domain contains one V‑type Ig-like domain and shows 45% aa identity with mouse NFAM1. The 86 aa cytoplasmic domain contains one ITAM consensus sequence.

• Long Name:

  NFAT Activating Protein with ITAM Motif 1

• Entrez Gene IDs:

  150372 (Human); 74039 (Mouse); 362966 (Rat)

• Alternate Names:

  calcinerin/NFAT-activating ITAM-containing protein; Calcineurin/NFAT-activating ITAM-containing protein; CNAIP; CNAIPbK126B4.4; FLJ40652; NFAM1; NFAT activating protein with ITAM motif 1; NFAT activation molecule 1; NFAT-activating protein with ITAM motif 1