• Species Reactivity

  Human

• Specificity

  Detects human RSK4 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) RSK1, rhRSK2, or rhRSK3 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 374521

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human RSK4    
  Met1-Leu745    
  Accession # Q9UK32

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RSK4

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases frequently activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSK4, also known as RPS6KA6, is commonly deleted in patients with complex X-linked mental retardation. Unlike other RSKs, RSK4 is constitutively active in serum-starved cells, and may participate in non-growth factor signaling.

• Long Name:

  Ribosomal Protein S6 Kinase IV

• Entrez Gene IDs:

  27330 (Human); 67071 (Mouse); 317203 (Rat)

• Alternate Names:

  EC 2.7.11; EC 2.7.11.1; p90-RSK 6; p90RSK6; pp90RSK4; PP90RSK4,90 kDa ribosomal protein S6 kinase 6; ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 4; RPS6KA6; RSK4; RSK-4; RSK4ribosomal protein S6 kinase, 90kD, polypeptide 6; S6K-alpha 6; S6K-alpha-6