• Species Reactivity

  Feline

• Specificity

  Detects feline CXCR4. Stains feline CXCR4 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2A Clone # 374606

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with feline CXCR4    
  Met1-Ser353    
  Accession # P56498

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  Feline peripheral blood mononuclear cells

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  Immersion fixed feline peripheral blood mononuclear cells

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR4

CXCR4, also known as fusin, is a 7TM G protein-linked chemokine receptor. CXCR4 is expressed on B cells, monocytes, CD4⁺ T cells, colonic epithelial cells, and astrocytes and binds SDF-1/CXCL12. CXCR4 is up‑regulated on mammary tumors. CXCR4, in cooperation with CD134 and heparan sulfate proteoglycans, functions as a co-receptor for feline immunodeficiency virus (FIV) entry into cells. Feline CXCR4 shares 97%, 95%, 88%, and 90% aa sequence identity with canine, human, mouse, and rat CXCR4, respectively.

• Entrez Gene IDs:

  7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

• Alternate Names:

  CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor