• Species Reactivity

  Human

• Specificity

  Detects human FPRL2. Stains human FPRL2 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2B Clone # 374822

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human FPRL2    
  Met1-Met352    
  Accession # P25089

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  NS0 mouse myeloma cell line transfected with human FPRL2    
   

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRL2/FPR3

FPRL2, also known as FMLP receptor-like 2, is a 40 kDa 7TM protein that is expressed on monocytes and dendritic cells. It interacts with formyl and acetyl peptides to attract these cells to sites of infection. FPRL2 signaling also inhibits DC maturation. Human FPRL2 shares 63% and 62% amino acid sequence identity with mouse and rat FPRL2, respectively.

• Long Name:

  Formyl Peptide Receptor-like 2

• Entrez Gene IDs:

  2359 (Human); 14294 (Mouse)

• Alternate Names:

  FMLP-R-II; FMLPY; formyl peptide receptor 3; Formyl peptide receptor-like 2FMLP-related receptor II; FPR3; FPRH1; FPRH1FPRH2; FPRH2; FPRL2; FPRL2FML2_HUMAN; N-formyl peptide receptor 3; RMLP-R-I