• Species Reactivity

  Human

• Specificity

  Detects human TAPP1 in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG    _2A Clone # 376409

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TAPP1    
  Pro2-Val404    
  Accession # Q9HB21

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human TAPP1

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAPP1

TAPP1, gene name PLEKHA1 (pleckstrin homology family A, member 1) is a 70‑75 kDa cytoplasmic adaptor protein that is membrane-associated after binding of the phosphoinositide, PtdIns(3,4)P2. The 404 aa human TAPP1 shares 76% aa identity with mouse TAPP1. It is expressed most highly in skeletal muscle, spleen, lung, thymus and placenta. TAPP1 interacts with cytoskeletal syntrophins, colocalizing in membrane ruffles following PDGF treatment in transfected NIH‑3T3 cells. TAPP1 and the related TAPP2 appear to play a role in B and T cell activation.

• Long Name:

  Tandem PH Domain Containing Protein 1

• Entrez Gene IDs:

  59338 (Human); 101476 (Mouse); 361659 (Rat)

• Alternate Names:

  family A (phosphoinositide bindingspecific) member 1; PH domain-containing family A member 1; pleckstrin homology domain containing, family A (phosphoinositide bindingspecific) member 1; PLEKHA1; TAPP1