• Species Reactivity

  Human

• Specificity

  Detects human Spinesin in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 376620

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Spinesin    
  Tyr27-Leu457 (Phe369Leu)    
  Accession # Q0P514

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Spinesin (Catalog # ) under non-reducing conditions only

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acids 1‑49), a transmembrane domain (amino acids 50‑70), a stem region and a serine protease domain (amino acids 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (amino acids 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The amino acid sequence of human Spinesin is 94%, 82%, 81% and 79% identical to that of chimpanzee, canine, rat and mouse.

• References:

1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.

2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.

3. Carninci, P. et al. (2000) Genome Res. 10:1617.

4. Shibata, K. et al. (2000) Genome Res. 10:1757.

• Entrez Gene IDs:

  80975 (Human); 80893 (Mouse)

• Alternate Names:

  EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5