• Species Reactivity

  Human

• Specificity

  Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants in flow cytometry.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 591853

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human GIPR    
  Met1-Cys466    
  Accession # P48546

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GIPR

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

• Long Name:

  Gastric Inhibitory Polypeptide Receptor

• Entrez Gene IDs:

  2696 (Human); 381853 (Mouse); 25024 (Rat)

• Alternate Names:

  gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2