• Species Reactivity

  Human

• Specificity

  Detects human Fucosyltransferase 3/5 (FUT3/5) in Western blots. The amino acid sequences of recombinant human FUT3 and recombinant human FUT5 are 91% identical.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 425037

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Fucosyltransferase 3    
  Arg35-Thr361    
  Accession # P21217

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # )    
  Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 3/5 (FUT3/5)

Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha -4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa 1 to 15), a transmembrane region (aa 16 to 34) and an ectodomain (aa 35 to 361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken.

• Entrez Gene IDs:

  2525 (Human)

• Alternate Names:

  Fucosyltransferase 3/5