• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse SerpinI1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross reactivity with recombinant mouse Serpin I1 is observed and no cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A12, B6, B8, B9, C1, D1, F2, G1, or I2 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 437016

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Serpin I1    
  Ala19-Leu410    
  Accession # AAH18043

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human and Mouse Serpin I1

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin I1

Serpin I1, also known as neuroserpin, is a secreted serine protease inhibitor predominantly expressed in the brain. Serpin I1 defects cause familial encephalopathy with neuroserpin inclusion bodies (FEN1B), an autosomal dominant dementia. The amino acid sequence of human Serpin I1 is 99%, 93%, 88%, 86% and 80% identical to that of chimpanzee, dog, rat, mouse and chicken.

• Entrez Gene IDs:

  5274 (Human); 20713 (Mouse); 116459 (Rat)

• Alternate Names:

  DKFZp781N13156; Neuroserpin; Peptidase inhibitor 12; PI12; PI-12; PI12Serpin I1; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1; Serpin I1; serpin peptidase inhibitor, clade I (neuroserpin), member 1