• Species Reactivity

  Human

• Specificity

  Detects human GPR37L1. Stains human GPR37L1 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2A Clone # 444532

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human GPR37L1    
  Accession # O60883

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37L1

GPR37L1 (G-protein coupled receptor 37-like 1), also called ETBR-LP-2 (endothelin B receptor-like protein 2), is a 481 aa, orphan 7-transmembrane receptor with calculated molecular weight of 53 kDa. GPR37L1 is expressed in cerebellar Bergmann glia, cerebral cortex and internal capsule fibers in the central nervous system. It shows 39% aa identity with GPR37, a neuronal protein that accumulates in juvenile Parkinson’s disease neurons under endoplasmic reticulum stress. GPR37L1 does not bind known endothelin B family members. The extracellular portions of human and mouse GPR37L1 share 85% aa identity.

• Long Name:

  G Protein-coupled Receptor 37-like 1

• Entrez Gene IDs:

  9283 (Human); 171469 (Mouse); 252939 (Rat)

• Alternate Names:

  endothelin B receptor-like protein 2; ET(B)R-LP-2; ETBRLP2; ETBR-LP-2; ETBR-LP-2endothelin type b receptor-like protein 2; G protein-coupled receptor 37 like 1; GPR37L1; G-protein coupled receptor 37 like 1; G-protein coupled receptor 37-like 1