Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Benefits  

• Rolls out of bottle for easy cell culture medium preparation

• Eliminates time spent aliquoting

• One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?  

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

What are ProDots® Proteins?  

ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.

• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.    et al. (1989) Exp. Hematol.     17:116. The ED    ₅₀ for this effect is 10-60 pg/mL.

• Source

  E. coli-derived Thr31 - Pro204, with an -N-terminal Met

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  18.8 kDa

Data Images

Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

• Long Name:

  Granulocyte Colony Stimulating Factor

• Entrez Gene IDs:

  1440 (Human); 12985 (Mouse)

• Alternate Names:

  C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin