• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD27 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhFas Ligand, rhTRAIL R3, rhTRAIL R4, rhLT beta R, rhNGF-R1, rh4-1BB, rhGITR, rhTNFR1, rhTNF RII, rhBAFF, rhTAJ, rhEDA, rhOPG, rhRELT, rhRANK, recombinant mouse (rm) CD30, rmCD40, rmGITR, rmHVEM, rmRANK, rmFas, rmTRAIL R2, rm4-1BB, rmLT beta R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, recombinant rat Fas, or recombinant feline Fas.

• Source

  Monoclonal Rat IgG    _2A Clone # 137910

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7    
  Thr21-Arg182    
  Accession # P41272

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27 is a lymphocyte-specific member of the tumor necrosis factor receptor superfamily (TNFRSF) and is designated TNFRSF7 (1, 2). Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region (3). Mouse and human CD27 share approximately 65% amino acid identity. CD27 exists as homodimers on the cell surface via an extracellular disulfide bond in the membrane-proximal region. A soluble form of CD27 is also produced during the immune response and is found in various body fluids (4). CD27 is expressed on subsets of T and B cells. The expression of CD27 is upregulated upon T-cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells (5). CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (1, 2). Ligation of CD27 on B cells has been shown to inhibit terminal differentiation of activated mouse B cells into plasma cells and enhances commitment to memory B cell responses (5).

• References:

1. Croft, M. (2003) Nature Reviews Immunol. 3:609.

2. Croft, M. (2003) Cytokine and Growth Factor Reviews 14:265.

3. Gravestein, L.A. et al. (1993) Eur. J. Immunol. 23:943.

4. Lens, S.M. et al. (1998) Semin. Immunol. 10:491.

5. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

• Entrez Gene IDs:

  939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

• Alternate Names:

  CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7