• Species Reactivity

  Mouse

• Specificity

  Detects mouse Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human Progranulin is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 333731

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Progranulin/PGRN    
  Thr18-Leu589    
  Accession # P28798

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Progranulin (Catalog # ) under non-reducing conditions only.     We recommend (Catalog #    ) for Western blot applications.  

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it belongs to none of the well-established growth factor families (4). Mouse Progranulin is 589 amino acids (aa) in length and contains a 17 aa signal sequence and a 572 aa mature region that has four potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VXCX_5-6CX₅CCX₈CCX₆CCXDX₂HCCPX₄CX_5-6CX₂ (1). Progranulin is secreted in an intact form (2, 4) or undergoes proteolysis leading to the release of multiple peptides made from the seven tandem repeats, the granulins (5‑7). Mouse Progranulin shares 87% and 75% aa sequence identity with rat and human Progranulin, respectively. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), development synovium of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation plus of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers, and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4, 5, 12, 14‑20).

• References:

1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073. 

2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863. 

3. Liu, Y. et al. (2007) BMC Cancer 7:22. 

4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347. 

5. Davidson, B. et al. (2004) Cancer 100:2139. 

6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331. 

7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993. 

8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510. 

9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727 

10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.

11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.

12. He, Z. et al. (2003) Nat. Med. 9:225.

13. Zhu, J. et al. (2002) Cell 111:867.

14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.

15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.

16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.

17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.

18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.

19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.

• Entrez Gene IDs:

  2896 (Human); 14824 (Mouse)

• Alternate Names:

  Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin