• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat  beta ‑Actin in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 937215

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Peptide containing a sequence at the N-terminus of human beta ‑Actin    
  Accession # P60709

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.01 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  0.1-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma  
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

• Entrez Gene IDs:

  60 (Human); 11461 (Mouse); 81822 (Rat)

• Alternate Names:

  ACTB; actin, beta; actin, cytoplasmic 1; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; PS1TP5-binding protein 1; PS1TP5BP1