• Species Reactivity

  Human

• Specificity

  Detects human IL-20 R alpha in direct ELISAs and Western blots. In Western blots, approximately 5‑15% cross‑reactivity with recombinant human (rh) IFN-gamma R2, rhIL-10 R, rhIL-10 R beta, rhIL-20 R beta, and rhIL-22 BP is observed and no cross-reactivity with rhIFN-gamma RI, recombinant mouse IL‑20 R alpha, or rhIL‑22 R is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 173714

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL-20 R alpha    
  Val30-Lys250    
  Accession # Q9UHF4

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-20 R alpha

IL-20 receptor alpha (IL-20 R alpha ), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).

The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).

IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3‑5).

• References:

1. Kotenko, S.V. (2003) Cytokine & Growth Factor Reviews 13:223.

2. Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.

3. Dumoutier, L. et al. (2001) J. Immunol. 167:3534.

4. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517s.

5. Sheikh. F. et al. (2004) J. Immunol. 172:2006.

• Long Name:

  Interleukin 20 Receptor alpha

• Entrez Gene IDs:

  53832 (Human); 237313 (Mouse)

• Alternate Names:

  class II cytokine receptor ZCYTOR7; CRF2-8; Cytokine receptor class-II member 8; Cytokine receptor family 2 member 8; FLJ40993; IL-20 R alpha; IL20R alpha; IL-20R1IL-20 receptor subunit alpha; IL20RA; IL-20Ra; IL-20R-alpha; interleukin 20 receptor, alpha; interleukin-20 receptor I; interleukin-20 receptor subunit alpha; zcytor7