• Species Reactivity

  Human

• Specificity

  Detects human IL-37/IL-1F7 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-36Ra/IL-1F5, rhIL-36 alpha/IL-1F6, rhIL-36 beta/IL-1F8, or rhIL-18/IL-1F4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 261506

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human IL-37/IL-1F7    
  Lys27-Asp192    
  Accession # NP_775297

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human IL-18 R alpha /IL-1 R5 Fc Chimera (Catalog # ) to immobilized Recombinant Human IL-37/IL-1F7 (Catalog # ) coated at 100 ng/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-37/IL-1F7

Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).

• References:

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.

2. Kumar S. et al. (2000) J. Biol. Chem. 275:10308.

3. Busfield S.J. et al. (2000) Genomics 66:213.

4. Kumar S. et al. (2002) Cytokine 18:61.

5. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.

6. Gao, W. et al. (2003) J. Immunol. 170:1077.

7. Pan, G. et al. (2001) Cytokine 13:1.

8. Sims, J.E. et al. (2001) Trends Immunol. 22:533.

• Long Name:

  Interleukin 37

• Entrez Gene IDs:

  27178 (Human)

• Alternate Names:

  FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein