• Species Reactivity

  Mouse

• Specificity

  Detects mouse TORC3 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human TORC3 and no cross-reactivity with recombinant mouse (rm) TORC1 or rmTORC2 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 643729

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse TORC3    
  Leu153-Pro275    
  Accession # Q91X84

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TORC3

TORC3, also known as CREB-regulated transcription coactivator 1 (crtc3), is an approximately 80 kDa protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC3 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1 transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1 transcription. Alternate splicing of mouse TORC3 generates isoforms that lack the N-terminal 299 amino acids (aa). Within aa 153-275, mouse TORC3 shares 99% and 98% aa sequence identity with human and rat TORC3, respectively.

• Long Name:

  Transducer of Regulated CREB-binding Protein 3

• Entrez Gene IDs:

  64784 (Human); 70461 (Mouse); 365297 (Rat)

• Alternate Names:

  CREB regulated transcription coactivator 3; CRTC3; FLJ21868; TORC3; TORC-3; TORC3CREB-regulated transcription coactivator 3; Transducer of CREB protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; Transducer of regulated cAMP response element-binding protein 3; transducer of regulated CREB protein 3