• Species Reactivity

  Human

• Specificity

  Detects human Insulin in ELISA.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 873724

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  The construct consists of the alpha mating factor signal followed by a 10 aa peptide leader, B chain aa 1-29, AAK spacer A chain aa 1-21.

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

• Entrez Gene IDs:

  3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)

• Alternate Names:

  IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin